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. 2012 Nov 5;13:595. doi: 10.1186/1471-2164-13-595

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Copyright ©2013 Liu et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Evaluation of sequencing depth for the assembly of the catfish transcriptome assembly. (A) Assemblies were evaluated based on the number of assembled contigs with length ≥ 200 bp and 1 kb. The X-axis represents assemblies with various sequencing depths generated by CLC Genomics Workbench, left Y-axis represents the number of contigs with length ≥ 200 bp in thousand, and right Y-axis represents the number of contigs with length ≥ 1 kb in thousand. (B) Assemblies were evaluated based on the number of zebrafish proteins that were identified in the assembled catfish contigs. The X-axis represents assemblies with various sequencing depths assembled by CLC Genomics Workbench, left Y-axis represents the percentage of zebrafish proteins that can be detected in catfish and right Y-axis represents the percentage of zebrafish proteins that can be detected in catfish with match length ≥ 90%.