Figure 12.

PRE mutant designs. TESS predictions of transcription factor binding sites (Table 1) were used to design oligomers for deleting one or more putative transcription factor binding sites in the PRE. A. Wildtype PRE sequence with all target sites indicated by rectangles corresponding to TF positions. B-H. Mutant oligomers with specific target sites indicated by rectangles corresponding to TF positions. TF sites introduced by mutagenesis have boldface and underlined names. Specific base mutations indicated by boldface and underline in sequences.