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Southwestern blot of PRE vs. cell line nuclear extracts. Nuclear extracts from cell lines NB (lane 1), PC12 (lane 2), HeLa (lane 3), and HeLa treated with TPA (lane 4) were separated on 10% SDS-PAGE and transferred to nitrocellulose membrane. Membrane was probed with radiolabeled PRE oligomers. Blot photographs were size-equalized by use of the same lot of protein standards. Major bands are indicated with solid arrows. Minor bands are indicated with dotted-line arrows.
