Figure 5.

ELISA of PRE variant CAT reporter clones in NB and PC12 cells. Functional differences of PRE mutant clones (M1-M7) from wildtype were assayed in NB and PC12 cells. A. pβXbB were constructed and transfected into NB or PC12 cell cultures as described in the main text. Cultures were harvested and analyzed by CAT ELISA. ELISA signals were normalized to “wildtype” values and subject to Dunnett’s multiple t. Means significantly different at p > 0.05 indicated by “*”. B. ELISA of samples from NB cell cultures. C. ELISA of samples from PC12 cell cultures. Data presented is back-transformed from statistical analysis. Asymmetrical error bars and Dunnett’s t limits are due to Box-Cox transformation, when used.