Table 10.
Mutations induced in the PRE
| Variant | Sites deleted | Sites created |
|---|---|---|
| Wildtype PRE |
|
|
| M1 |
AP2, GATA1, GC Box (II)a, PuF, R, SP1 (I)b |
AML1a |
| M2 |
AP2, GC Box (I), GATA1, Pax4α, PuF, SP1 (I) |
AML1a/AML1c, AP4, GT-IIBα |
| M3 |
R, TCERG (I)c |
|
| M4 |
GATA1/GATA2, SP1 (II) |
GR, PR |
| M5 |
R, TCERG (I) |
YY1 |
| M6 |
TCERG1 (II) |
GATA1/GATA2 |
| M7 | TCERG1 (I, II), R |
aTwo distinct GC boxes were identified in the wildtype PRE sequence and are designated as “I” and “II”.
bTwo distinct SP1 sites in addition to the GC boxes were identified in the wildtype PRE sequence and are designated as “I” and “II”.
cTwo distinct TCERG1 sites were identified in the wildtype PRE sequence and are designated as “I” and “II”.