Table 3.
Adjusted/normalized ELISA and EMSA signals in NB cells and extracts
|
Variant |
EMSAa |
|
||||
|---|---|---|---|---|---|---|
| |
Band I |
Band II |
ELISAa |
|||
| Rel. PRE = 0 | g | Rel. PRE = 0 | g | Rel. PRE = 1 | g | |
| PRE |
0.00 ± 0.18 |
0.00 ± 0.82 |
0.00 ± 0.11 |
0.00 ± 0.82 |
1.00 ± 0.17 |
0.00 ± 0.82 |
| M1 |
1.81c ± 0.24 |
3.24 ± 0.99/0.95 |
−1.76c ± 0.11 |
−4.86 ± 1.29/1.31 |
0.37 ± 0.05 |
−3.12 ± 0.92/0.96 |
| M2 |
−1.33b ± 0.26 |
−2.39 ± 0.79/0.84 |
−0.43 ± 0.13 |
−1.18 ± 0.62/0.66 |
0.81 ± 0.12 |
−0.91 ± 0.60/0.63 |
| M3 |
0.60 ± 0.27 |
1.07 ± 0.65/0.61 |
0.79b ± 0.23 |
2.19 ± 0.80/0.76 |
1.69b ± 0.13 |
3.40 ± 1.02/0.98 |
| M4 |
0.40 ± 0.38 |
0.72 ± 0.62/0.59 |
−1.62b ± 0.09 |
−4.48 ± 1.21/1.23 |
0.89 ± 0.02 |
−0.52 ± 0.58/0.60 |
| M5 |
−0.28 ± 0.21 |
−0.51 ± 0.58/0.60 |
0.07 ± 0.22 |
0.19 ± 0.58/0.57 |
0.34b ± 0.08 |
−3.24 ± 0.95/0.99 |
| M7 |
0.27 ± 0.31 |
0.48 ± 0.60/0.58 |
−0.61 ± 0.12 |
−1.68 ± 0.68/0.73 |
0.16b ± 0.12 |
−4.14 ± 1.13/1.16 |
| M7 |
1.00 ± 0.42 |
1.78 ± 0.74/0.70 |
0.87b ± 0.35 |
2.41 ± 0.84/0.79 |
1.04 ± 0.09 |
0.18 ± 0.58/0.57 |
| |
F (p) |
Ψd |
F (p) |
Ψ |
F (p) |
Ψ |
| ANOVA | 9.79 (< 0.01) | 1.65 ± 0.82/0.62 | 26.54 (< 0.01) | 2.72 ± 1.09/0.97 | 21.20 (< 0.01) | 2.43 ± 1.01/0.88 |
aData is mean ± standard error of the mean (SEM). EMSA is normalized to wildtype = 0. ELISA is relative to wildtype = 1. SEM reported as (x/y as upper/lower) is asymmetrical due to noncentrality of standardized effect size measures [43].
bSignal significantly different from wildtype at p < 0.05.
cELISA and EMSA signal both significantly different from wildtype at Bonferroni-adjusted p < 0.05.
dΨ as estimate ± 95% confidence interval.