Table 4.
Adjusted/normalized ELISA and EMSA signals in PC12 cells and extracts
|
Variant |
EMSAa |
|
||||
|---|---|---|---|---|---|---|
| |
Band I |
Band II |
ELISAa |
|||
| Rel. PRE = 0 | g | Rel. PRE = 0 | g | Rel. PRE = 1 | g | |
| PRE |
0.00 ± 0.23 |
0.00 ± 0.82 |
0.00 ± 0.1 |
0.00 ± 0.82 |
1.00 ± 0.11/0.09 |
0.00 ± 0.82 |
| M1 |
1.25 ± 0.92/0.78 |
1.77 ± 0.74/0.69 |
−1.38c ± 0.07 |
−5.56 ± 1.44/1.45 |
0.39c ± 0.02 |
−6.84 ± 1.73 |
| M2 |
−0.91 ± 0.11 |
−1.43 ± 0.65/0.69 |
−0.14 ± 0.18 |
−0.56 ± 0.58/0.60 |
0.67 ± 0.05/0.04 |
−2.05 ± 0.74/0.78 |
| M3 |
0.56 ± 0.07 |
0.84 ± 0.63/0.60 |
1.20b ± 0.14 |
4.82 ± 1.30/1.28 |
0.83 ± 0.18/0.12 |
−0.8 ± 0.59/0.62 |
| M4 |
0.61 ± 0.48/0.44 |
0.91 ± 0.63/0.60 |
−1.04c ± 0.11 |
−4.20 ± 1.15/1.17 |
0.45c ± 0.01 |
−5.33 ± 1.39/1.4 |
| M5 |
0.16 ± 0.12 |
0.25 ± 0.58/0.57 |
0.30 ± 0.07 |
1.23 ± 0.67/0.63 |
0.43b ± 0.04/0.03 |
−5.69 ± 1.47/1.48 |
| M6 |
0.27 ± 0.06 |
0.41 ± 0.59/0.58 |
−0.25 ± 0.13 |
−1.01 ± 0.61/0.64 |
0.43b ± 0.01 |
−5.68 ± 1.47/1.48 |
| M7 |
1.46 ± 0.58/0.52 |
2.04 ± 0.78/0.73 |
1.58b ± 0.19 |
6.36 ± 1.62 |
1.03 ± 0.03 |
0.12 ± 0.58/0.57 |
| |
F (p) |
Ψd |
F (p) |
Ψ |
F (p) |
Ψ |
| ANOVA | 4.23 (< 0.01) | 1.09 ± 0.78/0.45 | 58.96 (< 0.01) | 4.05 ± 1.49/1.42 | 29.96 (< 0.01) | 2.89 ± 1.14/1.03 |
aData is mean ± standard error of the mean (SEM). EMSA is normalized to wildtype = 0. ELISA is relative to wildtype = 1. SEM reported as (x/y as upper/lower) is asymmetrical due to noncentrality of standardized effect size measures [43].
bSignal significantly different from wildtype at p < 0.05.
cELISA and EMSA signal both significantly different from wildtype at Bonferroni-adjusted p < 0.05.
dΨ as estimate ± 95% confidence interval.