Table 8.
Oligomers for EMSA of PRE-derived mutants
| Oligomer | Forward (F) 5′ to 3′a | Reverse (R) 5′ to 3′ |
|---|---|---|
| PRE (WT) |
5′-TCGAGTGCCGAGCGGGGTGGGCCGGATCAG-3′ |
5′-CTGATCCGGCCCACCCCGCTCGGCACTCGA-3′ |
| M1 |
5′-TCGAGTGCCGAGCGGGGTGGTACCGATCAG-3′ |
5′-CTGATCGGTACCACCCCGCTCGGCACTCGA-3′ |
| M4 |
5′-TCGAGTGCCGAGCGGGGTGGGCCTGTACAG-3′ |
5′-CTGTACAGGCCCACCCCGCTCGGCACTCGA-3′ |
| M2 |
5′-TCGAGTGCCGAGCGCTGTGGGCCGGATCAG-3′ |
5′-CTGATCCGGCCCACAGCGCTCGGCACTCGA-3′ |
| M3 |
5′-TCGAGTCGCGAGCGGGGTGGGCCGGATCAG-3′ |
5′-CTGATCCGGCCCACCCCGCTCGCGACTCGA-3′ |
| M4 |
5′-TCCATGGCCGAGCGGGGTGGGCCGGATCAG-3′ |
5′-CTGATCCGGCCCACCCCGCTCGGCCATGGA-3′ |
| M6 |
5′-TCGAGTGCCGATCGGGGTGGGCCGGATCAG-3′ |
5′-CTGATCCGGCCCACCCCGATCGGCACTCGA-3′ |
| M7 | 5′-TCGAGTCCGGAGCGGGGTGGGCCGGATCAG-3′ | 5′-CTGATCCGGCCCACCCCGCTCCGGACTCGA-3′ |
aUnderlined bases have been mutated from wildtype PRE.