Figure 4. ERK-1/2 partially mediates the effect of NDP-α-MSH on PPAR expression.

Cells were treated for 15 min with 10µM PD98059 and then 0.1 µM NDP-α-MSH was added to the culture medium. Cells were harvested after 24 h of incubation and PPAR protein levels were assessed by western blot. (A) The effect of NDP-α-MSH over PPAR-γ expression was not observed in the presence of PD98059 in astrocytes. (B) Similarly, the effect of NDP-α-MSH on PPAR-β levels was not observed in the presence of PD98059 in microglial cells. Data were analyzed by one sample t Test and are expressed as mean ± SEM. **p<0.01 vs. control group.