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. 2013 Feb 26;8(2):e57303. doi: 10.1371/journal.pone.0057303

Figure 2. N. benthamiana plants transformed with the BvSTI gene.

Figure 2

A) N. benthamiana 11-4 and 11-6 plants transformed with the BvSTI gene and normal untransformed plant. B) Southern blot of N. benthamiana plants. Lane 1–5, BvSTI transformant 11-4, 11-5, 11-6, 11-13 and 12-2, respectively; lane 6, untransformed normal control plant, lane 7, BvSTI plasmid DNA. All DNAs were digested with the NdeI restriction enzyme. M1, DNA molecular weight markers in kilobasepairs (Kb). C) RT-PCR analysis of transformants 11-4, 11-5, 11-6, 11-13 and 12-2 (lane 1–5, respectively) and normal untransformed control (lane 6) with BvSTI gene specific primers that amplify a 0.6 Kb gene fragment. Actin, housekeeping actin gene amplification with gene specific primers that generate a 0.54 Kb gene fragment (lane 1–6) used as quality and quantity control of the RNA being analyzed. M2, 100-bp DNA molecular weight markers.