Figure 6. DNA cleavage assays with topoisomerase VI screen hits.
A. Assaying the abilities of screen hits to induce M. mazei topo VI-mediated DNA cleavage with 1 unit topo VI (50 nM). B. Inhibition of S. shibatae topo VI by screen hits. C. Assaying the abilities of screen hits to induce S. shibatae topo VI-mediated DNA cleavage. D. Protection of DNA from ADPNP-induced, S. shibatae topo VI-mediated cleavage by screen hits.