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. 2012 Sep 26;8:178. doi: 10.1186/1746-6148-8-178

Table 1.

Pens of cattle sampled in Alberta Canada in 2007 with 1 fecal grab sample and 1 hide swab collected per steer at each sampling and PFGE performed on samples positive for E. coli O157:H7

Feedlot A B Total
Pen
 1
 2
 3
 4
 5
 6
 7
 1
 2
 3
 4
  
cattle (n)
 153
 152
 153
 253
 258
 155
 233
 151
 235
 138
 106
 1987
Sample 1 date
 May 22
 Jun 4
 Jul 20
 Jul 27
 Sep 26
 Oct 9
 Oct 23
 Jul 6
 Jul 12
 Aug 10
 Aug 30
  
  SS fecal isolates (n)
 2
 36
 12
 19
 7
 3
 0
 19x
 44
 10
 1
 153
  SS perineal swabs (n)
 2
 30
 11
 17
 3
 3
 0
 17
 40
 7
 1
 131
  LS fecal isolates (n)
 13
 37
 100
 54
 24
 2
 1
 36
 107
 20
 31
 425
Sample 2 date
 Sep 24
 Sep 6
 Oct 3
 Oct 17
 Dec 10
 Nov 29
 Dec 6
 Sep 28
 Sep 28
 Nov 14
 Nov 27
  
SS fecal isolates (n)
 1
 0
 0
 0
 0
 0
 0
 8x
 0
 0
 0
 9
  SS perineal swabs (n)
 0
 0
 0
 0
 0
 0
 0
 6
 0
 0
 0
 6
  LS fecal isolates (n)
 3
 10
 0
 2
 3
 0
 1
 40
 9
 3
 0
 71
  LS fecal isolates from former SS (n)
 0
 1
 0
 2
 2
 0
 0
 7
 9
 0
 0
 21
Total isolates tested by PFGE (n)
 21
 113
 123
 92
 37
 8
 2
 126
 200
 40
 33
 795
Total REPC (n)
 1
 12
 14
 14
 6
 1
 1
 20
 13
 6
 6
 85y
Total unique isolates (n)
 8
 33
 11
 14
 12
 6
 0
 13
 17
 8
 11
 86y
SS fecal & SS perineal swab in same REPC (n) 1 1 0 0 0 0 NAz 0 0 0 0 2

Numbers of super–shedders (SS)v and low- shedders (LS)w within pens. Numbers of, restriction endonuclease digestion clusters (REPC, 90% or greater similarity) and unique isolates (< 90% similarity) within and across pens.

vSuper-shedder, shedding at least 104 CFU E. coli O157:H7/g feces.

wLow-shedder, shedding < 104 CFU E. coli O157:H7/g feces.

xOne steer was a super-shedder at both samplings.

yTotal comparing all isolates across pens.

zNA, not applicable, no super-shedders in pen.