Table 2.
Titres of antibodies correlate with the extent of disease in mice
| Group | Antibodya | IgG reactivityb | Disease activityc | ||||
|---|---|---|---|---|---|---|---|
| |
|
0d |
7 |
14 |
0 |
7 |
14 |
| 1 |
R a-murine BP180/ CXVII |
0 |
128±13 |
232±30.3 |
0 |
0 |
1.5±0.17 |
| 2 |
NR IgG |
0 |
0 |
0 |
0 |
0 |
0 |
| 3 |
S a-murine BP180/ CXVII |
0 |
640±149 |
1248±310 |
0 |
1±0.15 |
2.5±0.17 |
| 4 | NS IgG | 0 | 0 | 0 | 0 | 0 | 0 |
a Adult mice were injected every other day with 10mg of either rabbit Ig (group 1, n=10) or sheep IgG (group 3, n=10) against murine BP180/ CXVII. Control groups received similar amounts of control rabbit (group 2, n=6) or sheep (group 4, n=6) antibodies. R a-, rabbit anti-; NR, normal/control rabbit; S a-, sheep anti-; NS, normal/control sheep.
b End-point titre of IgG reactivity to the basal membrane was assayed by indirect IF on mouse skin sections.
c The extent of skin disease (disease activity) was scored as follows: 0, no lesions; 1, < 10 lesions or < 1% of the skin surface; 2, > 10 lesions or 1-5% of the skin surface; 3, 5-10%; 4, 10-20%; and 5, >20% involvement of the skin surface.
d Disease activity and IgG titres are presented at as mean ± s.e.m. at day 0, 7 and 14, respectively. Spearman’s rank correlation test showed significant correlation between antibody titres as detected by IF and disease activity in mice receiving rabbit (group 1) or sheep (group 3) pathogenic antibodies.