Figure 4. Lymphocyte-deficient K14E7 ear skin is more susceptible to immune-mediated rejection.

(A) Representative flow cytometry plots of CD45 and CD3 staining in ear skin derived from K14E7, K14E7×RAG1^−/− and RAG1^−/− mice. Haematoxylin and eosin staining of ear skin tissue sections taken from K14E7 and K14E7×RAG1^−/− mice are shown. (B) A graph summarizing the proportion of CD45.2⁺CD3⁺ cells seen in each of the representative plots from (A). Data represents 3–5 individual mice/group from 2 independent experiments (C) Ear skin from K14E7, K14E7×RAG1^−/− and RAG1 mice was grafted onto the flank of C57BL/6 mice and the percent of mice with surviving grafts was plotted against time post grafting. The K14E7 and K14E7×RAG1^−/− grafting groups contained 16 mice/group while the RAG1^−/− group contained 4 mice from at least 2 independent experiments.