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. 2012 Jun 12;41(3):839–848. doi: 10.3892/ijo.2012.1517

Figure 2.

Figure 2

Tob1 induces apoptosis in gastric cancer cells. (A) Cells were transfected as mentioned above and were subjected to flow cytometric analysis upon staining with Annexin V and propidium iodide (PI). Right panel shows statistical analysis of apoptosis in cells transfected with control vector or myc-Tob1. *p<0.05 and ***p<0.001, compared to respective control vector. (B) The caspase-3 activity was assessed from MKN28 and AGS cells transfected with myc-Tob1 as indicated above. **p<0.01 and ***p<0.001, compared to control vector. (C) The expression of Bax, Bcl-2, and cleavage of PARP after transfection of empty vector or myc-Tob1 was investigated using their specific antibodies. Actin was used as loading control. (D) MKN28 and AGS cells transfected with empty vector or myc-Tob1 were subjected to flow cytometry to analyze cell cycle distribution.