Table I.
Primer sequences for quantitative RT PCR.
| Gene | Orientation | Sequences | Tm (°C) |
|---|---|---|---|
| Tob1 | Forward | 5′-TCTGTATGGGCTTGGCTTG-3′ | 54.9 |
| Reverse | 5′-TGTTGCTGCTGTGGTGGT-3′ | 54.3 | |
| CDKN2B | Forward | 5′-CAACGGAGTCAACCGTTTC-3′ | 54.9 |
| Reverse | 5′-GGTGAGAGTGGCAGGGTCT-3′ | 54.2 | |
| Cyclin D1 | Forward | 5′-GAAGATCGTCGCCACCTG-3′ | 56.6 |
| Reverse | 5′-GACCTCCTCCTCGCACTTCT-3′ | 59.5 | |
| uPAR | Forward | 5′-ACACCACCAAATGCAACGA-3′ | 52.7 |
| Reverse | 5′-CCCCTTGCAGCTGTAACAC-3′ | 57.1 | |
| PPARδ | Forward | 5′-GGGAAAAGTTTTGGCAGGAG-3′ | 55.4 |
| Reverse | 5′-TGCCCAAAACACTGTACAACA-3′ | 53.9 | |
| HPRT | Forward | 5′-CTCAACTTTAACTGGAAAGAATGTC-3′ | 54.1 |
| Reverse | 5′-TCCTTTTCACCAGCAAGCT-3′ | 55.5 |