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. Author manuscript; available in PMC: 2014 Feb 21.
Published in final edited form as: Immunity. 2013 Jan 31;38(2):360–372. doi: 10.1016/j.immuni.2013.01.007

Figure 2. TSLP enhances murine Th9 cell generation.

Figure 2

Mouse splenic naïve CD4+ T cells were isolated and differentiated to Th9 and Th2 cells in the presence or absence of 10 ng/ml TSLP.

(A) Intracellular staining of IL-9-positive and IL-13-positive population in Th9 and Th2 cell cultures assessed by flow cytometry.

(B) Il9 and Il13 expression in Th9 and Th2 cell cultures was determined by qPCR.

(C) ELISA analysis of IL-9 and IL-13 secreted by Th9 and Th2 cells, respectively.

(D) Intracellular staining (left) or ELISA analysis (right) of Th9 cell cultures incubated with increasing doses of TSLP.

(E) Tslpr expression in Th subsets was determined by qPCR.

(F) Tslpr expression in Th9 cells and Th2 cells incubated with or without TSLP.

(G) Il9 expression in Th2 or Th9 cell cultures, incubated with or without TSLP. Data are representative of 3–4 experiments and are presented as the mean ± SEM of at least 3 replicate samples. *, p<0.05. WT, wild type BALB/c cells; KO, BALB/c TSLPR-deficient cells.