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. Author manuscript; available in PMC: 2014 May 22.

Published in final edited form as: Mol Cell Endocrinol. 2012 Nov 5;371(0):62–70. doi: 10.1016/j.mce.2012.10.011

Fig 3 — Effects of a low dose ACTH injection (4ng, iv) on the levels of ACTH [A] and corticosterone [B] in plasma, and adrenal zona fasciculata levels of heteronuclear (hn, ) and messenger (m, ) RNA levels for StAR [C], cytochrome P450 side chain cleavage (CYP11A) [D], melanocortin type 2 receptor (MC2R) [E] and melanocortin receptor associated protein (MRAP) [F]. Rats were killed by decapitation at the time points indicated. Plasma ACTH levels tended to increase at 5 min. This small and non-statistically significant increase in plasma ACTH injection caused significant increases in plasma corticosterone levels, accompanied by significant increases in StAR, CYP 11A and MRAP hnRNAs, and StAR and MRAP mRNAs. No changes in MC2R mRNA were observed following the ACTH injection. Data points represent the mean and SE of the values in 3 to4 rats per group. The arrows show the time of ACTH injection. *P<0.01; **P<0.005 compared to time 0. Figure modified from data in Spiga et al, 2011a

Inline graphic — Effects of a low dose ACTH injection (4ng, iv) on the levels of ACTH [A] and corticosterone [B] in plasma, and adrenal zona fasciculata levels of heteronuclear (hn, ) and messenger (m, ) RNA levels for StAR [C], cytochrome P450 side chain cleavage (CYP11A) [D], melanocortin type 2 receptor (MC2R) [E] and melanocortin receptor associated protein (MRAP) [F]. Rats were killed by decapitation at the time points indicated. Plasma ACTH levels tended to increase at 5 min. This small and non-statistically significant increase in plasma ACTH injection caused significant increases in plasma corticosterone levels, accompanied by significant increases in StAR, CYP 11A and MRAP hnRNAs, and StAR and MRAP mRNAs. No changes in MC2R mRNA were observed following the ACTH injection. Data points represent the mean and SE of the values in 3 to4 rats per group. The arrows show the time of ACTH injection. *P<0.01; **P<0.005 compared to time 0. Figure modified from data in Spiga et al, 2011a