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. Author manuscript; available in PMC: 2014 May 22.

Published in final edited form as: Mol Cell Endocrinol. 2012 Nov 5;371(0):62–70. doi: 10.1016/j.mce.2012.10.011

Fig 4 — Time-relationship between levels of StAR hnRNA (A), cytoplasmatic phospho-TORC2 (pTORC2, ) and nuclear dephospho TORC2 (dpTORC2, ) protein (B), and nuclear phospho-CREB (pCREB) (C), in the adrenal zona fasciculata of rats subjected to restraint stress Rats were subjected to restraint stress for 1h (indicated by the black bar) and killed by decapitation at the time points indicated. Significant increases in nuclear TORC2 preceded the increases in StAR hnRNA. Significant increases in nuclear phospho-CREB levels paralleled to increases in StAR hnRNA. Data points represent the mean and SE of the values in 4 rats per group. *, p<0.05; **, p<0.01; ***, p<0.001. D shows representative western blots for cytoplasmic pTORC2, nuclear dpTORC2 and pCREB. Beta actin and histone deacetylase (HDAC) were used as controls for cytoplasm and nuclear proteins, respectively.

Inline graphic — Time-relationship between levels of StAR hnRNA (A), cytoplasmatic phospho-TORC2 (pTORC2, ) and nuclear dephospho TORC2 (dpTORC2, ) protein (B), and nuclear phospho-CREB (pCREB) (C), in the adrenal zona fasciculata of rats subjected to restraint stress Rats were subjected to restraint stress for 1h (indicated by the black bar) and killed by decapitation at the time points indicated. Significant increases in nuclear TORC2 preceded the increases in StAR hnRNA. Significant increases in nuclear phospho-CREB levels paralleled to increases in StAR hnRNA. Data points represent the mean and SE of the values in 4 rats per group. *, p<0.05; **, p<0.01; ***, p<0.001. D shows representative western blots for cytoplasmic pTORC2, nuclear dpTORC2 and pCREB. Beta actin and histone deacetylase (HDAC) were used as controls for cytoplasm and nuclear proteins, respectively.