Table 1.
Immunophenotype analysis for passage 3 stem cells from lean and obese mice. In response to obesity, sqASCs and IFP cells did not significantly alter surface marker expression. Interestingly, however, obesity significantly increased percentage of the MSCs expressing PDGFRα but a trend toward to decrease CD105 level. Results from 3 independent experiments for PDGFRα and CD105 in lean and obese MSCs with mean ± SEM displayed (n ≥ 3 mice per experiment. Values with different superscript letters are significantly different; p < 0.05). For other cell types, results averaged from of 2 independent experiments (n ≥ 3 mice per experiment).
| Surface Marker | MSCs | sqASCs | IFP cells | |||
|---|---|---|---|---|---|---|
| Lean | Obese | Lean | Obese | Lean | Obese | |
| CD11b | ≤ 0.1% | ≤ 0.1% | ≤ 0.1% | ≤ 0.1% | ≤ 0.5% | ≤ 0.3% |
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| CD45 | ≤ 0.1% | ≤ 0.1% | ≤ 0.1% | ≤ 0.1% | ≤ 0.3% | ≤ 0.3% |
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| TER119 | ≤ 0.1% | ≤ 0.1% | ≤ 0.3% | ≤ 0.3% | ≤ 0.3% | ≤ 0.1% |
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| ||||||
| CD31 | ≤ 0.1% | ≤ 0.1% | ≤ 0.1% | ≤ 0.1% | ≤ 0.3% | ≤ 0.3% |
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| CD34 | ≤ 0.5% | ≤ 0.5% | 1.95% | ≤ 0.5% | 1.65% | 1.27% |
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| Sca-1 | ≥ 99% | ≥ 99% | ≥ 99% | ≥ 99% | ≥ 99% | ≥ 99% |
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| CD44 | > 95% | > 95% | 90% | 61% | > 95% | > 95% |
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| PDGFRα | 68.9±6%a | 82.7±7%b | 27.3% | 27.5% | 95% | 93.5% |
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| CD105 | 68.9±11%a | 31.3±10%a | 64.2% | 60.8% | ≥ 85% | ≥ 85% |