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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: J Biol Inorg Chem. 2013 Jan 19;18(3):289–297. doi: 10.1007/s00775-012-0973-1

FIG. 4.

FIG. 4

Introducing a threonine at residue 41 disrupts Fe(III)-Met-80 coordination. a. Fe(III)-Met-80 coordination was measured by monitoring absorbance from 600-800 nm with 250 μM ferricyt c in 50 mM sodium phosphate pH 7.5. b. Nuclear magnetic resonance spectroscopy at pH 7 (sodium phosphate) and pH 5.2 (sodium acetate). Downfield heme methyl resonances are labeled M (His/Met), K (His/Lys) and H (His/His) as assigned in [36]. Upfield Met-80 ε-CH3 group is labeled ε-CH3.