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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: J Biol Inorg Chem. 2013 Jan 19;18(3):289–297. doi: 10.1007/s00775-012-0973-1

FIG. 5.

FIG. 5

Changes at residue 41 alter the pKa of the alkaline transition. The 695 nm absorbance of 250 μM ferricyt c in sodium phosphate (50 mM) at 25°C was monitored as the pH was increased. Curves represent fit to a two-state transition.