. Author manuscript; available in PMC: 2014 Mar 1.

Published in final edited form as: J Biol Inorg Chem. 2013 Jan 19;18(3):289–297. doi: 10.1007/s00775-012-0973-1

Table 1.

Rates of apoptosome activation and biophysical properties of cyt c variants.

	Apoptosome activation		CD	DSC	Redox potential	Met-80 ε- C¹H₃	Alkaline transition
	pmol.min⁻¹.min⁻¹	Normalized to WT	T_m (°C)	T_m (°C)	mV ± SD	ppm	pK_a
PBS	0.003 ± 0.001	0.015
WT	0.200 ± 0.003	1	71 ± 3	66.7 ± 0.2	226 ± 3	−23.9	9.3 ± 0.4
G41S	0.317 ± 0.004	1.58	69 ± 1	66.6 ± 0.8	227 ± 4	−23.2	7.8 ± 0.3
G41A	0.181 ± 0.004	0.9	69 ± 1	67.9 ± 0.5	244 ± 3	−23.5	8.1 ± 0.5
G41T	0.014 ± 0.001	0.07	68 ± 4	67.6 ± 0.5	195 ± 1	−19.9	6.7 ± 0.2