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. Author manuscript; available in PMC: 2014 Mar 27.
Published in final edited form as: Toxicol Lett. 2013 Jan 21;218(1):81–90. doi: 10.1016/j.toxlet.2013.01.009

Fig. 6.

Fig. 6

Independence of HBCDD action on basal Leydig cell steroidogenesis of COX-2 expression and intracellular calcium. (A) Effects of celecoxib (5 μM and 15 μM), a COX-2 inhibitor, on in vitro steroidogenesis. Cells were treated during 6 h with 10 μM HBCDD in the absence and presence of celecoxib (B) HBCDD effects on Leydig cell basal and hCG (0.125 ng/ml)-supported androgen production when challenged with BAPTA-AM (20 μM), an intracellular Ca2+ chelator. Notice that BAPTA-AM did not diminish HBCDD toxic effects. Data points represent means ± SEM in one out of three similar experiments. * p < 0.05 vs. corresponding control (0).