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. 2012 Jun 28;41(3):995–1004. doi: 10.3892/ijo.2012.1536

Figure 4.

Figure 4

Effect of RasGRF1 knockdown on migration and adhesion of ARMS cells. (A), ARMS cell chemotaxis after down-regulating RasGRF1 expression. Chemotaxis of RH30-derived cells across Transwell membranes covered with gelatin in response to SDF-1 (300 ng/ml), I-TAC (100 ng/ml), HGF (100 ng/ml), IGF-II (100 ng/ml), and insulin (10 ng/ml) gradients. Black bars show chemotaxis of control RH30scr cells, while white bars represent chemotaxis of RH30 cells with down-regulated expression of RasGRF1. Data from 4 separate experiments are pooled together. *p<0.05 compared to unstimulated controls (−). (B), ARMS cell adhesion after down-regulating RasGRF1 expression. Adhesion of human RMS cells to fibronectin after stimulation by SDF-1 (300 ng/ml), I-TAC (100 ng/ml), HGF (100 ng/ml), IGF-II (100 ng/ml), and insulin (10 ng/ml). Data from 3 separate experiments are pooled together. *p<0.05 as compared to unstimulated controls (−).