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Lung squamous cell carcinoma stained for TMPRSS4 and CAIX. Frozen lung tumor tissue sections were co-stained with rabbit polyclonal anti-TMPRSS4 and mouse monoclonal anti-CAIX antibodies and then with secondary species-specific antibodies; Cy3-conjugated goat anti-rabbit (red) and Cy2-conjugated anti-mouse (green). Nuclei were stained with DAPI (blue). For negative controls, tissue sections were stained with rabbit and mouse IgG isotype antibodies.
