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. 2013 Feb 27;5:1. doi: 10.3389/fnsyn.2013.00001

Figure 1.

Figure 1

LTP2 and LTP3 persistence and magnitude is reduced with gelonin in the patch pipette. (A) Pictorial representation of the TBS protocol used to induce LTP1, 2, and 3. When more than one train was used (i.e., 4TBS and 8TBS) there was a 30 s inter-train interval. All TBS trains were delivered at the test-pulse intensity. (B) Mean percent change in EPSC amplitude following 4TBS and 8TBS (C) in control cells and cells filled with the membrane impermeable translation inhibitor gelonin (geloninin). Inset shows example EPSC traces before (black) and immediately after (gray) delivery of 4TBS or 8TBS in the control situation. Scale bar indicates 200 μA and 10 ms. Inclusion of gelonin in the patch pipette significantly reduced LTP magnitude at 160 min post-4TBS (D) and post-8TBS (E) (*p < 0.05). Gelonin did not affect EPSC recordings in the absence of TBS (A) nor did it affect weak LTP1 induced by 1TBS (F) or LTP2 and LTP3 when applied extracellularly (geloninout) (B and C).