Figure 3.

Enhanced presynaptic function associated with LTP2 is dependent on postsynaptic translation. (A) Scatter plot showing the half-life of FM 1-43 destaining versus distance to filled dendrite for individual terminals in slices stimulated with 4TBS with and without gelonin in the patch pipette. (B) Histogram summarizing destaining rate data from the “Control” group in (A) and from “No TBS” slices with distances binned. Destaining rate was enhanced in slices stimulated with 4TBS and there was no effect of distance to nearest postsynaptic dendrite in these untreated cells. (C) Histogram of data obtained from 4TBS gelonin-treated group in (A) and “No TBS” slices treated with gelonin, with distances binned. Inclusion of gelonin in the patch pipette abolished enhanced release induced by 4TBS only at sites close to (0–0.5 μm) the filled postsynaptic cell, but had no effect on release in the absence of TBS (One-Way ANOVA, ^*p < 0.05). (D) Examples of destaining from two representative puncta in the 0.0–0.5 μm distance group. i, control terminal with no gelonin in the pipette shows normal, LTP-related enhanced rate of dye release. ii, terminal with gelonin in the postsynaptic cell shows reduced rate of dye release.