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. 2012 Jul 4;41(3):876–884. doi: 10.3892/ijo.2012.1545

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Copyright © 2012, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Phenotypic differences in the CD138⁺ and CD138⁻ fraction of KYMM-2 cells. (A) KYMM-2 was fractionated into CD138⁺ and CD138⁻ populations using CD138 magnetic beads. The purity of each fraction was approximately 90%. (B) Gene expression between the two fractions was analyzed by real-time RT-PCR. Higher expression of SDC1, IRF4 and PRDM1 was observed in CD138⁺ fraction (p<0.01, Student’s t-test), while higher expression of BCL6 observed in CD138⁻ fraction (p<0.01). (C) An increase in Annexin V positive cells was observed at 40 μM of lenalidomide in the CD138⁺ fraction compared with CD138⁻ fraction.