Figure 2. Non-methylated islands are associated with gene promoters in vertebrate genomes.
(A) A histogram depicting the proportion of protein-coding transcription start sites (TSSs) which are overlapped by an NMI for all seven species. Blue bars indicate overlap with annotated TSSs and red bars indicate overlap with additional TSSs identified using RNA-seq data (platypus, chicken and lizard) or Xtev gene sets (frog). (B) Profiles of non-methylated DNA were plotted over a 6-kb window centred on all TSSs with an NMI (dark blue), without an NMI (blue), and for all transcription termination sites (TTS, black). The non-methylated DNA signal peaks at the TSS of gene promoters in all vertebrates.