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. 2013 Feb 15;450(Pt 2):417–425. doi: 10.1042/BJ20121599

Figure 2. Nucleic acid contamination of purified TtPilF.

Figure 2

(A) UV absorption spectrum of TtPilF (continuous line) and BSA (broken line), both at 0.1 mg/ml, recorded in 25 mM Tris/HCl (pH 8.0), 100 mM NaCl and 10 mM MgCl2. (B) Agarose gel (1.5%) electrophoresis of TtPilF. A total of 50 μg of purified TtPilF was loaded in each lane. Lane 1 has TtPilF without pretreatment, lane 2 has TtPilF after treatment with 2 units of DNase (RNase-free) and lane 3 has TtPilF after treatment with 2 units of RNase (DNase-free). DNA markers are indicated to the left of the gel.