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. 2013 Mar 1;140(5):1100–1110. doi: 10.1242/dev.088989

Fig. 2.

Fig. 2.

Tsh and Tio expression in Drosophila tubules. (A-F) Tsh (red, A-C) and Tio (black, D-F) expression in embryonic MpTs. Tsh is first detected in SCs at stage 13 (B, arrow) and maintained throughout embryogenesis. Tio is first detected in PCs from stage 11 (D, arrows) and in SCs from stage 13 (E). By stage 16, Tio expression levels in SCs are high but diminished in PCs (F). (G,H) Low- (G) and high- (H) magnification views of stage 16 embryo showing Tsh (red) and Tio (green, overlap appears yellow) co-expression in SCs (arrows). (I-L) Tsh (red; I,J) and Tio (red; K,L) expression in larval (third instar; I,K) and adult (J,L) MpTs. Tsh but not Tio is expressed in larval and adult SCs. PCs are marked with anti-Cut (green, A-C) and SCs with c724>CD8GFP (green, I-L). Tubule outline is marked with broken lines. Scale bars: 50 μm in A-F; 50 μm in G,H; 30 μm in I-L.