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. 2013 Mar 1;140(5):1100–1110. doi: 10.1242/dev.088989

Fig. 5.

Fig. 5.

tsh is required for SC morphology. (A-D′) Adult MpTs, SCs marked with c724>UAS-CD8GFP (green in A-D, white in A′-D′); tubules counterstained for actin (phalloidin, red). (A,B) Control tubules showing ‘stellate’-shaped SCs in main segment (A,A′) and ‘bar’-shaped cells in initial segment (B,B′). (C,D) tsh knockdown tubules have uncharacteristically round SCs in main (C,C′) and initial (D,D′) segments. (E,F) Three-dimensional reconstruction of control (E) and tsh knockdown (F) SCs shown from different angles. (G) Surface area and volume measurements for control (n=4) and tsh knockdown (n=4) SCs. (The reduction in volume accounts for only a 20% reduction in surface area in tsh knockdown SCs.) Scale bars: 30 μm in A-D; 10 μm in A′-D′,E,F.