Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Mar 1;140(5):1111–1122. doi: 10.1242/dev.080226

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

PMC Copyright notice

Fig. 4. — Prosp-NH-derived FGFs stimulate outgrowth of endothelial processes. (A-G) Transverse (B-F) and sagittal (G) sections through chick NH at planes indicated in A. D,E show serial adjacent sections. Arrowheads and arrows point to endothelial processes/axons outside (arrowheads) or within/beneath (arrow) ventral NH. (H-I) [CAMv] isolation from wild-type or Roslin-GFP embryos. (J-K) Short radial projections emerge from [CAMv] explants cultured alone for 48 hours. Whole-mount immunolabelling shows that these are Tie1⁺ (J′). Dotted circles in J′ outline explant and ring of short processes. (L-L″) [CAMv]-E4 prosp-NH co-culture after 24 (L) and 40 hours (L′). Arrows indicate long endothelial processes. (L′″) Quantitative analysis of long processes from proximal versus distal faces of [CAMv] explants. Error bars represent s.e.m. ^***P<0.001. d, distal; p, proximal. (M,M′) High-power views, after sectioning; extending processes are DAPI⁺VE-cadherin⁺Tie1⁺. (N-R′) GFP [CAMv] explants cultured with PBS- (N), Fgf10- (O,P) or Fgf3- (Q) soaked beads for 42 hours. FGF-beads promote significant numbers of long projections from proximal faces of [CAMv] explants. Analyses of wild-type co-cultures reveals that endothelial projections are DAPI⁺VE-cadherin⁺Tie1⁺ (R,R′). (S,S′) Wild-type [CAMv] explant cultured with Fgf10 for 40 hours. Whole-mount immunohistochemical analyses show many long (>90 μm) Tie1⁺ projections. Dotted circles show explant outline and reference for control process lengths (see J′). (T,U) Transverse sections through [CAMv] explants cultured with/without Fgf10. No significance difference is detected in DAPI⁺ or pH3⁺ cells. (V,V′) Transverse sections through prosp-NH explants pre-soaked in anti-Fgf3 and anti-Fgf10 antibodies. Fgf10 and Tbx2 are expressed normally (compare with Fig. 2D). (W) Prosp-NH explant pre-soaked in anti-Fgf3 and anti-Fgf10 fails to provoke long process outgrowth from GFP [CAMv] explants, which appear similar to controls (compare with K). Righthand panel shows quantitative analysis. Scale bars: 70 μm in A-E,G-L″,N-Q,S,S′,V,W; 45 μm in T,U; 25 μm in M,M′,R,R′; 20 μm in F.