Table 2.
Effect of freshwater clam extract and soy protein isolate on body weight gain, food intake, organ weight, serum parameters, fecal steroids, and hepatic CYP7A1 transcription rate in ratsa.
| Control groupb | FCE groupb | SPI groupb | |
|---|---|---|---|
| Mean ± SEM | Mean ± SEM | Mean ± SEM | |
| Body weight gain (g) | 71.3 ± 3.4ab | 77.3 ± 2.9b | 64.8 ± 2.2a |
| Food intake (g/d)c | 14.7 ± 0.6 | 13.3 ± 0.5 | 14.1 ± 0.5 |
| Relative organ weight (g/100 g of body weight) |
|||
| Liver | 3.40 ± 0.08b | 3.44 ± 0.06b | 2.66 ± 0.04a |
| Epididymal adipose tissue | 1.52 ± 0.05 | 1.37 ± 0.05 | 1.10 ± 0.24 |
| Serum lipids (mmol/L) | |||
| Total cholesterol | 2.28 ± 0.10b | 1.56 ± 0.06a | 1.56 ± 0.04a |
| Triglyceride | 0.65 ± 0.04 | 0.57 ± 0.03 | 0.65 ± 0.02 |
| Serum adiponectin (ng/mL) | 4426 ± 293a | 5562 ± 476a | 7212 ± 310b |
| Dry fecal weight (g for 3d)d | 1.2 ± 0.1a | 3.2 ± 0.3c | 1.7 ± 0.0b |
| Fecal steroids (μmol/3 days) | |||
| Total neutral sterol | 29.5 ± 3.6a | 119 ± 10b | 42.5 ± 2.8a |
| Total bile acids | 36.3 ± 4.4a | 247 ± 18b | 51.7 ± 3.2a |
| Hepatic transcription rate (arbitrary units)e | |||
| CYP7A1 | 100 ± 19a | 303 ± 47b | 152 ± 26a |
aEach value is the mean with its standard error for 6 rats in each dietary group. The statistical significance of differences among values was analyzed by ANOVA and then by Tukey's multiple-range test. Values in a row with different letters indicate a statistically significant difference, P < .05.
bControl group: basal diet; FCE group: freshwater clam extract-supplemented diet; SPI group: soy protein isolate-supplemented diet.
cFood intake was measured from day 2 to day 3.
dFeces were collected over the final 3 days of the experimental period.
eTranscription rate was estimated by the pre-mRNA level.