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Effects of resveratrol, p-coumaric acid, and arbutin on human TYR activity determined using the lysates of HEMs. The lysates of HEK293 cells, HEK293-TYR cells, and HEMs were subjected to western blotting for TYR, TRP1, TRP2, and GAPDH (a) and in vitro TYR activity assay (b). The lysates of HEMs were used in the human TYR activity assay (c). Data are presented as percentages of uninhibited activities (means ± SE, n = 3).
