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. 2013 Feb 12;2013:813091. doi: 10.1155/2013/813091

Figure 3.

Figure 3

Long-term exposure of mast cells to CSM inhibits allergic degranulation. BMMCs were cultured in regular culture medium, in the presence of CSM (1.5%) or LPS (100 ng/mL) in the third week of bone marrow culture. Then, cells were sensitized with DNP-specific IgE, followed by activation with dinitrophenyl-conjugated human ovalbumin (DNP-Ova). Degranulation was assessed by the release of β-hexosaminidase in the supernatants from cells. Data are mean ± SEM of quadruplicate samples (n = 4).