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Testicular fine-needle aspiration (TEFNA). A 23-gauge fine needle attached to a 10-mL syringe coupled to a Cameco syringe holder is percutaneously inserted into the testicle to map different areas. Negative pressure is applied, and the needle is moved in and out within the testis with no change in direction. A tissue fragment from each mapped area is expelled into a pre-identified tube containing sperm culture medium.
