Table 1.
E. coli strains
| Strains | Genotype/Relevant characteristics | Source |
|---|---|---|
| OP50 | OP50 ura | [37] |
| RNAi control strain | HT115(DE3) W3110 rnc14::Tn10 (DE3 PlacUV5-T7 polymerase) | [46] |
| aroD mutant | HT115(DE3) aroD717::IS1 | This study |
| Keio collection WT | BW25113 | [16] |
| Keio collection aroD | BW25113 aroD | [16,36], This study |
| OP50 Su resistant | OP50 R26 | This study; [47] |
| aroD mutant/ugt-27 RNAi | HT115(DE3)aroD717::IS1/L4440(ugt-27) | This study; [38] |
| RNAi control + vector | HT115(DE3)/pMMB67EH | This study |
| aroD mutant + vector | HT115(DE3)aroD717::IS1/pMMB67EH | This study |
| mutant + aroD | HT115(DE3)aroD717::IS1/pMMB67EH[aroD] | This study |
| ubiG mutant | GD1 ubiG | [15] |
| ubiG mutant + rescue | GD1 ubiG/pAHG(ubiG+) | [15] |