LPS-induced motility is inhibited by histamine
viaH4receptor activation. (A)
Upon an inflammatory challenge triggered by LPS (100 ng/ml), 100 μM
histamine inhibited migration, an effect involving H4R activation.
In the presence of histamine, only when H4R was blocked (Ant
H4R: JNJ7777120, 5 μM), LPS-induced migration was restored.
(B) Representative photomicrographs depict the inhibitory effect of
histamine on LPS-induced motility upon H4R activation. Data are
expressed as mean ± SEM (n = 3-20) and as
mean total number of cells migrating across the scratch wound
(*p < 0.05, **p < 0.01,
***p < 0.001, using Bonferroni’s multiple
comparison test).