Mesenchymal stem cell (MSC) viability and proliferation depend on hydrogel reticulation and hyaluronic acid concentration. MSCs were cultivated for 28 days at 4×105 cell/mL, in hydrogels of different compositions of hyaluronic acid (HA):gelin (40:60)%, (50:50)%, or (60:40)%, respectively, containing three different extralinker concentrations (1:4, 1:6, or 1:8). Cell viability was examined using Alamar blue test. Values are represented as (mean±standard error of the mean) normalized per day one results (n=4).