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. 2012 Oct 24;19(4):288–298. doi: 10.1089/ten.tec.2012.0144

FIG. 2.

FIG. 2.

Cellular density and hydrogel reticulation affect MSC proliferation and morphology. Viability and phenotype of MSCs cultivated within (HA: gelin [40:60], extralinker ratio 1:8) at different cellular concentrations (1×105, 2×105 or 4×105 cells/mL), and two hydrogel crosslinking ratios 1:6 (A, C) and 1:8 (B, D) for 1, 7, 14, 21, and 28 days. Viable MSCs in a hydrogel containing extralinker ratio 1:6 (A) or extralinker ratio 1:8 (B); reticulation reagents were evaluated using Alamar blue test. Results are given as means±standard error of the mean. Light microscope shows encapsulated MSC morphology and network formation, at various cell densities and crosslinking concentration 1:6 (C) and 1:8 (D) over 21 days in culture (magnification×10). (*) corresponds to p<0.05.