Figure 5.

The membrane interaction domains (MIDs) of GB virus type C E2 are essential for its inhibitory effect on human immunodeficiency virus type 1 (HIV-1) Gag processing and release. A, Schematic of immunoglobulin G (IgG)–E2 and mutant constructs. B, Gag-Pol was expressed in the presence of E2, IgG-E2, or truncated IgG-E2 constructs (E2DN1, E2DN2, E2DC2, and E2DMID) by transfection of 293T cells. Cells were harvested and lysed 2 days after transfection to analyze processing of the Gag-Pol precursor by Western blot analysis. C, E2, IgG-E2, or the E2DMID mutant was cotransfected with Gag-Pol into 293T cells. Two days after transfection, Western blotting was used to assess processing of HIV-1 Gag and virus-like particle release.