Table 2.
Field-identification of arboviruses, bloodmeals, and mosquito genera using the ArboChip3.0 in Lopburi and Kamphaeng Phet, Thailand, April to March, 2011
| Sample number | Arthropod species (pool size) | Collection method* | location | Microarray identification† | ||
|---|---|---|---|---|---|---|
| Virus | Blood- meal | Mosquito genus | ||||
| Th9-0011 | Cx. quinquefasciatus (1) | BA | House | CxFV | Human | Culex |
| Th9-0024 | Ae. aegypti (1) | BA | House | NA | NA | Aedes |
| Th9-0042 | Armigeres subalbatus (1) | BA | House | NA | NA | Armigeres |
| Th9-0047 | Cx. quinquefasciatus (10) | BA | House | NA | Human | Culex |
| Th9-0122 | Ae. aegypti (1) | BA | House | DENV-3 | DNP | Aedes |
| Th9-0164 | Cx. tritaeniorhynchus (24) | LT | Farm | JEV | NA | Culex |
| Th9-0167 | Ae. albopictus (1) | LT | Farm | NA | NA | Aedes |
| Th9-0175 | Cx. tritaeniorhynchus (25) | LT | Farm | JEV | NA | Culex |
| Th9-0235 | Cx. tritaeniorhynchus (25) | BA | Cow pen | NA | Mammal§ | Culex |
| Th9-0278 | Mansonia uniformis (6) | LT | Farm | NA | NA | Mansonia |
| Th9-0643 | Dermacentor species (1) | NA | Lab | NA | Dog | NA |
BA = backpack aspiration; LT = Centers for Disease Control and Prevention CO2 baited light trap.
DNP = did not prime; NA = not applicable; CxFV, Culex flavivirus; DENV-3, dengue virus type 3; JEV = Japanese encephalitis virus. Mosquito pools that were not suspected of being infected with flaviviruses by polymerase chain reaction (PCR) screening or not containing any bloodfed mosquitoes were not analyzed on the microarray for flavivirus or bloodmeal identification.
Mosquito contained a bloodmeal but did not amplify with mammalian or avian primers.
Bloodmeal PCR amplicon was identified as Bos indictus (brahman cow) Cytb by sequencing, cow-specific probes were not present on this microarray design.