Table 2.
Effects of mosquito pool size and time after intrathoracic injection (day PI) on detection of DENV by virus isolation, RNA detection, and NS1 antigen detection*
| Pool size | Day PI | Titer (log10 PFU/mL) | RT-PCR | Nested PCR | Mean A450 (range) |
|---|---|---|---|---|---|
| 1 | 1 | 1.94 | positive | positive | 5.18 (5.15–5.20) |
| 1 | 7 | 4.34 | positive | positive | 6.35 (6.27–6.45) |
| 1 | 14 | 4.72 | positive | positive | 6.17 (6.15–6.20) |
| 1 | 21 | 3.54 | positive | positive | 6.08 (5.98–6.16) |
| 1 | Uninfected | negative | negative | negative | 0.12 (0.12–0.12) |
| 10 | 1 | 1.72 | positive | positive | 6.08 (5.92–6.08) |
| 10 | 7 | 4.25 | positive | positive | 6.64 (6.27–7.35) |
| 10 | 14 | 3.49 | positive | positive | 6.12 (6.07–6.20) |
| 10 | 21 | 3.11 | positive | positive | 6.06 (6.06–6.06) |
| 10 | Uninfected | negative | negative | negative | 0.15 (0.15–0.17) |
| 100 | 1 | ND | negative | positive | 2.67 (2.24–2.98) |
| 100 | 7 | ND | positive | positive | 6.25 (6.12–6.33) |
| 100 | 14 | ND | positive | positive | 6.03 (6.02–6.06) |
| 100 | 21 | ND | positive | positive | 6.17 (6.07–6.29) |
| 100 | Uninfected | negative | negative | negative | 0.14 (0.14–0.14) |
| 500 | 1 | ND | positive | positive | 3.61 (3.40–3.85) |
| 500 | 7 | ND | positive | positive | 6.18 (6.11–6.23) |
| 500 | 14 | ND | positive | positive | 6.01 (6.00–6.01) |
| 500 | 21 | ND | positive | positive | 6.08 (5.97–6.17) |
| 500 | Uninfected | negative | negative | negative | 0.17 (0.17–0.17) |
| 1,000 | 1 | ND | negative | negative | 1.49 (1.24–1.49) |
| 1,000 | 7 | ND | negative | negative | 3.07 (3.00–3.75) |
| 1,000 | 14 | ND | negative | negative | 4.10 (4.09–4.10) |
| 1,000 | 21 | ND | negative | negative | 6.65 (6.63–6.65) |
| 1,000 | Uninfected | negative | negative | negative | 0.14 (0.14–0.14) |
*
Pools of 1 infected mosquito in the total pool size shown were assayed by virus titration, RT-PCR, nested PCR, and NS1 Ag detection for DENV infection.
DENV = dengue virus; PI = post-infection; PFU = plaque-forming units; RT-PCR = reverse transcription-polymerase chain reaction; ND = titer below level of detection; positive/negative = PCR product detected/not detected on gel.