Table 3.

Effects of drying infected mosquitoes on detection of DENV in mosquito pools by virus isolation, RNA detection, and NS1 antigen detection^*

No. of days dried	Pool size	Titer (log10 PFU/mL) by day PI					RT-PCR by day PI					Nested PCR by day PI					Mean A450 by day PI
		Uninfected	1	7	14	21	Uninfected	1	7	14	21	Uninfected	1	7	14	21	Uninfected	1	7	14	21
1	1	neg	0	1.94	0	0	neg	pos	pos	pos	pos	neg	pos	pos	pos	pos	neg	6.88	6.96	6.92	6.73
1	10	neg	0	1.64	0	0	neg	pos	pos	pos	pos	neg	pos	pos	neg	pos	neg	6.90	6.92	6.85	6.62
1	100	neg	0	0	0	0	neg	neg	neg	pos	pos	neg	pos	pos	pos	neg	neg	6.33	6.91	6.88	6.70
3	1	neg	0	1.64	1.94	0	neg	pos	pos	pos	pos	neg	pos	pos	pos	pos	neg	2.19	4.44	4.55	4.54
3	10	neg	0	1.12	0	0	neg	pos	pos	pos	pos	neg	pos	pos	pos	pos	neg	4.66	4.61	4.56	4.53
3	100	neg	0	0	0	0	neg	pos	pos	neg	pos	neg	pos	pos	pos	pos	neg	4.08	4.60	4.54	4.57
14	1	neg	0	0	0	0	neg	neg	pos	pos	pos	neg	pos	pos	pos	pos	neg	1.33	5.98	5.66	4.70
14	10	neg	0	0	0	0	neg	neg	neg	pos	pos	neg	pos	pos	pos	pos	neg	1.82	5.71	5.55	4.68
14	100	neg	0	0	0	0	neg	neg	neg	neg	neg	neg	pos	pos	pos	pos	neg	1.16	5.62	5.54	4.69
30	1	neg	0	0	0	0	neg	neg	pos	neg	pos	neg	pos	pos	neg	pos	neg	1.44	6.27	6.16	6.11
30	10	neg	0	0	0	0	neg	neg	pos	pos	pos	neg	neg	pos	pos	pos	neg	2.06	6.12	6.12	6.00
30	100	neg	0	0	0	0	neg	pos	pos	pos	pos	neg	pos	pos	pos	pos	neg	0.94	6.17	6.08	6.06

^*Mosquitoes at 1, 7, 14, or 21 days post-DENV infection were subjected to 1, 3, 14, or 30 days of drying at room temperature and pools of 1 infected mosquito in the total pool size shown were constructed and assayed by virus titration, RT-PCR/nested PCR or NS1 Ag detection for DENV infection.

DENV = dengue virus; PI = post-infection; PFU = plaque-forming units; RT-PCR = reverse transcription-polymerase chain reaction; pos = positive, neg = negative.