Table 3.
No. of days dried | Pool size | Titer (log10 PFU/mL) by day PI | RT-PCR by day PI | Nested PCR by day PI | Mean A450 by day PI | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Uninfected | 1 | 7 | 14 | 21 | Uninfected | 1 | 7 | 14 | 21 | Uninfected | 1 | 7 | 14 | 21 | Uninfected | 1 | 7 | 14 | 21 | ||
1 | 1 | neg | 0 | 1.94 | 0 | 0 | neg | pos | pos | pos | pos | neg | pos | pos | pos | pos | neg | 6.88 | 6.96 | 6.92 | 6.73 |
1 | 10 | neg | 0 | 1.64 | 0 | 0 | neg | pos | pos | pos | pos | neg | pos | pos | neg | pos | neg | 6.90 | 6.92 | 6.85 | 6.62 |
1 | 100 | neg | 0 | 0 | 0 | 0 | neg | neg | neg | pos | pos | neg | pos | pos | pos | neg | neg | 6.33 | 6.91 | 6.88 | 6.70 |
3 | 1 | neg | 0 | 1.64 | 1.94 | 0 | neg | pos | pos | pos | pos | neg | pos | pos | pos | pos | neg | 2.19 | 4.44 | 4.55 | 4.54 |
3 | 10 | neg | 0 | 1.12 | 0 | 0 | neg | pos | pos | pos | pos | neg | pos | pos | pos | pos | neg | 4.66 | 4.61 | 4.56 | 4.53 |
3 | 100 | neg | 0 | 0 | 0 | 0 | neg | pos | pos | neg | pos | neg | pos | pos | pos | pos | neg | 4.08 | 4.60 | 4.54 | 4.57 |
14 | 1 | neg | 0 | 0 | 0 | 0 | neg | neg | pos | pos | pos | neg | pos | pos | pos | pos | neg | 1.33 | 5.98 | 5.66 | 4.70 |
14 | 10 | neg | 0 | 0 | 0 | 0 | neg | neg | neg | pos | pos | neg | pos | pos | pos | pos | neg | 1.82 | 5.71 | 5.55 | 4.68 |
14 | 100 | neg | 0 | 0 | 0 | 0 | neg | neg | neg | neg | neg | neg | pos | pos | pos | pos | neg | 1.16 | 5.62 | 5.54 | 4.69 |
30 | 1 | neg | 0 | 0 | 0 | 0 | neg | neg | pos | neg | pos | neg | pos | pos | neg | pos | neg | 1.44 | 6.27 | 6.16 | 6.11 |
30 | 10 | neg | 0 | 0 | 0 | 0 | neg | neg | pos | pos | pos | neg | neg | pos | pos | pos | neg | 2.06 | 6.12 | 6.12 | 6.00 |
30 | 100 | neg | 0 | 0 | 0 | 0 | neg | pos | pos | pos | pos | neg | pos | pos | pos | pos | neg | 0.94 | 6.17 | 6.08 | 6.06 |
Mosquitoes at 1, 7, 14, or 21 days post-DENV infection were subjected to 1, 3, 14, or 30 days of drying at room temperature and pools of 1 infected mosquito in the total pool size shown were constructed and assayed by virus titration, RT-PCR/nested PCR or NS1 Ag detection for DENV infection.
DENV = dengue virus; PI = post-infection; PFU = plaque-forming units; RT-PCR = reverse transcription-polymerase chain reaction; pos = positive, neg = negative.