Table 4.

Detection of infectious DENV, DENV RNA, or NS1 antigen in mosquito pools for which the infected mosquito had been subjected to both drying and freeze-thaw cycles^*

Pool size	Titer (log10 PFU/mL)	RT-PCR/Nested PCR	Mean A450
1	ND	pos/pos	6.39
10	ND	pos/pos	6.37
100	ND	pos/pos	6.33
1,000	n/a	neg/neg	neg

^*At 21 days post-DENV infection, mosquitoes were subjected to 30 days of drying at room temperature followed by 5 cycles of freezing and thawing. Pools of 1 infected and treated mosquito in the total pool size shown were constructed and assayed by virus titration, RT-PCR/nested PCR or NS1 Ag detection for DENV infection.

DENV = dengue virus; PFU = plaque-forming units; RT-PCR = reverse transcription-polymerase chain reaction; ND = titer below the level of detection; n/a = pool not assayed; pos = positive, neg = negative.