Table 5.
No. of freeze-thaw cycles | Pool size | Titer (log10 PFU/mL) by day PI | RT-PCR by day PI | Nested PCR by day PI | Mean A450 by day PI | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Uninfected | 1 | 7 | 14 | 21 | Uninfected | 1 | 7 | 14 | 21 | Uninfected | 1 | 7 | 14 | 21 | Uninfected | 1 | 7 | 14 | 21 | ||
1 | 1 | neg | 1.94 | 4.49 | 3.64 | 3.64 | neg | pos | pos | pos | pos | neg | pos | pos | pos | neg | neg | 6.75 | 6.65 | 6.63 | 6.54 |
1 | 10 | neg | 1.00 | 4.25 | 3.64 | 3.11 | neg | neg | pos | pos | pos | neg | neg | pos | neg | pos | neg | 6.66 | 6.65 | 6.60 | 6.51 |
1 | 100 | neg | 0 | 0 | 0 | 0 | neg | pos | pos | pos | pos | neg | pos | pos | neg | pos | neg | 6.78 | 6.57 | 6.58 | 6.40 |
5 | 1 | neg | 1.94 | 4.41 | 3.64 | 2.94 | neg | pos | pos | neg | pos | neg | pos | pos | pos | pos | neg | 4.60 | 6.64 | 6.63 | 6.56 |
5 | 10 | neg | 1.64 | 4.11 | 3.64 | 0 | neg | pos | pos | pos | pos | neg | pos | pos | pos | pos | neg | 6.38 | 6.55 | 6.65 | 6.55 |
5 | 100 | neg | 0 | 0 | 0 | 0 | neg | pos | pos | pos | pos | neg | pos | pos | pos | pos | neg | 4.94 | 6.57 | 6.60 | 6.56 |
Mosquitoes at 1, 7, 14, or 21 days post-DENV infection were subjected to 1 or 5 freeze-thaw cycles and pools of 1 infected mosquito in the total pool size shown were constructed and assayed by virus titration, RT-PCR/nested PCR or NS1 Ag detection for DENV infection.
DENV = dengue virus; PI = post-infection; PFU = plaque-forming units; RT-PCR = reverse transcription-polymerase chain reaction; pos = positive, neg = negative.