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Measurement of the apparent dissociation rate of AF488-Fc–FcεRIα complexes in the presence of IgE inhibitors. Excess IgE inhibitors (FcεRIα or E2_79) were incubated with preformed ternary complexes (AF488-Fc–FcεRIα–Tb-Ab). A slow decay in TR–FRET is observed in the absence of inhibitors. The rate of dissociation measured in the presence of E2_79 is characteristic of the ability of E2_79 to accelerate the dissociation of prebound IgE-Fc.
