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. Author manuscript; available in PMC: 2014 Feb 6.
Published in final edited form as: J Am Chem Soc. 2013 Jan 25;135(5):1783–1795. doi: 10.1021/ja309078t

Figure 10. Dual imaging of H2O2 and Caspase 8 following lipopolysaccharide challenge.

Figure 10

(a) Representative image (45 min post-injection) of mice following injection of ascorbic acid (IP, 200 mg/kg in 30 μL of saline), LPS (IP, 3 mg/kg in 50 μL of saline), z-VD(OMe)-OPh (IP, 1 μmol in 20 μL of DMSO), and/or their respective vehicles (IP, 30 or 50 μL saline or 20 μL DMSO). Mice were treated with ascorbic acid or vehicle 4.5 h prior to imaging, LPS or vehicle 4 h prior to imaging, and z-VD(OMe)-OPh or vehicle 2 h prior to imaging. Imaging was started following injection of a mixture of PCL-2 and IETDC (IP, 0.05 μmol each, in 50 μL of 7:3 DMSO:PBS). (b) Total photon flux, 30–60 min post-injection, for mice injected with PCL-2 and IETDC, ± LPS, ± z-VD(OMe)-OPh, and ± ascorbic acid. Statistical analyses were performed with a two-tailed Student’s t-test. *P < 0.05 (B and D: n = 3–4) and error bars are ±SEM.